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Authors
Affiliations
1 Institute of Agricultural Genetics, Pham Van Dong Road, Tu Liem District, Hanoi, VN
2 International Centre for Genetic Engineering and Biotechnology, ICGEB Campus, Aruna Asaf Ali Marg, P.O. Box 10504, New Delhi 110 067, IN
3 VNU University of Science, No. 334 Nguyen Trai Road, Thanh Xuan District, Hanoi, VN
Source
Current Science, Vol 109, No 3 (2015), Pagination: 541-551
Abstract
Plants respond to the adverse environment by activat-ing a series of stress-inducible genes, including genes encoding transcription factors. The expression of these genes is regulated by a core DNA sequence con-tained in their promoter region called cis-acting element. The promoter region of several stress-responsive genes contains several stress-regulated cis-acting elements such as dehydration-responsive element, C-repeat, low-temperature-responsive element, NAC recognition sequence and ZFHD recognition sequence. In this study we isolated a cDNA for a transcription factor named nuclear LIM interactor-interacting fac-tor from rice cDNA library by yeast one-hybrid screening using two target sequences of 50 nucleotides derived from two stress-inducible promoters, JRC0528 and JRC0332, of cold-inducible genes OsZF1 and OsNAC6 respectively, as baits. The NLI-IF protein showed both DNA-binding and transcriptional activities in yeast experiments. Expression of OsNLI-IF was found to be induced by cold, heat, salt and drought stresses. The OsNLI-IF gene overexpressing transgenic tobacco plants showed improvement in drought tolerance. The present study emphasizes that OsNLI-IF could be useful for development of drought-tolerant transgenic crop plants.
Keywords
Rice, Stress-Inducible Gene, Transcription Factor, Transgenic Tobacco.
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